| Johnson Lab> Research> Molecular Animation Movies |
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| Here are some of the QuickTime movies made in the lab |
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| Bateriophage HK97 |
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| Size: 9.5MB |
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HK97 is shown to expand from Prohead II to Head II from the outside. The pentons that are located at the vertices are colored green, and the hexons are colored red and blue to delineate the two halves separated by the distinctive "skew". The four states used to derive the intermediate time points are Prohead II, Expansion Intermediate I, Expansion Intermediate III, and Head II |
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| Size: 10MB |
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This movie shows a cross-section of HK97 to illustrate the progressive thinning of the capsid wall and doubling of internal volume as a result of expansion. The cutaway view emphasizes the motion of the penton subunits (green) located at the vertices during the transition from EI-III to Head II. An extrusion motion of the pentons characterizes the final maturation steps, and affects the order in which cross-links can occur, as is described for the cross-link movie. In the last frame, the semi-transparent Prohead II cross-section is displayed again, showing that the magnitude of the conformational change would allow a Head II particle to completely envelope its precursor. |
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| Size: 9.5MB |
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| Size: 9.8MB |
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These two movies show the contortion like motion that an isolated icosahedral asymmetric unit undergoes during the capsid expansion process from views normal (*hkasu.mov*) and tangential (*hkside.asu*) to the capsid shell. The icosahedral 2-fold (oval), 3-fold (triangle) and 5-fold (pentagon) axes are displayed in the first and last sets of frames. The A domains are blue, P domains are red, N-arms are yellow, and E-loops are green. |
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| Cowpea Chlorotic Mottle Virus (CCMV) |
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| Size: 8MB |
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This movie illustrates the large conformational changes the native state CCMV virus particles (coat proteins) undergo to form swollen particles when the bound metal ions are removed or the pH is raised. |
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| Flock House Virus (FHV) |
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| Size: 5.5MB |
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This movie shows how the ordered dsRNA found in Flock House Virus Structure controlls the capsid architecture |
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| Nudaurelia Capensis Omega Virus (NωV) |
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| The following movies illustrate the maturation of the Virus Like Particles (VLPs) and presumably of the virus in going from spherical Procapsid form via two known intermediate states (at pH 5.8 and at pH 5.5 respectively) to the final more compact and angular capsid form, at various levels of subunit organization: |
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| Size: 9.2MB |
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The cross-sectional view of VLPs showing large rearrangements in quaternary and tertiary structure of the Virus Like Particles (VLPs) during the process of maturation. |
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| Size: 10.6MB |
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Large scale rearragements illustrated using only the Ig like domains of the virus |
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| Size: 6.7MB |
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Following the movements of the 4 independent subunits of the icosahedral asymmetric unit of the virus (from the surface of the virus) |
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| Size: 5.6MB |
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The 4 independent subunits of the icosahedral asymmetric unit of the virus during the maturation process (viewing tangential to the virus surface) |
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| Size: 15.3MB |
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A close-up view of the virus capsid organization as the maturation process continues |
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